SubtiBank SubtiBank
Version comparison:

2017-05-22 16:12:562025-04-06 05:00:30

title

yocI

recQ

description

ATP-dependent DNA helicase, acts together with YrvE

ATP-dependent DNA helicase, acts together with [[protein|RecJ]] in replication fork maintenance

locus

BSU19220

BSU_19220

geneLength

1773

1776

function

DNA repair

replication fork maintenance

ec

outlinks

bsu

BSU19220

BSU_19220

The protein

Paralogous protein(s)

[[protein|RecS]]

[[this]]

Biological materials

Mutant

MGNA-B414 (yocI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1413 NBRP B. subtilis, Japan]

GP3543 (Δ[[gene|recQ]]::kan), available in [SW|Jörg Stülke]'s lab

MGNA-B414 (yocI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1413 NBRP B. subtilis, Japan]

BKE19220 (Δ[[gene|recQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT

BKK19220 (Δ[[gene|recQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT, downstream forward: _UP4_TAAAAAAACAGAAAAACAAT

References

Reviews

22933559

11497238, 22933559, 15217989, 32286623

References

Original publications

25246477

25246477, 25243132, 31675066, 17853894

The protein

Catalyzed reaction/ biological activity

unwinds covalently closed ds DNA (in complex with [[protein|SsbA|SSB]]) [pubmed|10360177]

[[protein|RecQ ]]controls [[protein|TopB|Topo III]] (de)catenation activity [pubmed|11497238,10360177]

ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

The protein

Protein family

[SW|helicase family] (according to UniProt)

The protein

[SW|Domains]

[SW|Helicase ATP-binding domain] (aa 27-196) (according to UniProt)

[SW|Helicase C-terminal domain] (aa 216-367) (according to UniProt)

HRDC domain (aa 513-591) (according to UniProt)

The protein

Structure

[PDB|4Q47] ([[protein|RecQ]] from Deinococcus radiodurans, 40% identity) [pubmed|25243132]

The protein

[SW|Localization]

associated with the [SW|replisome] [pubmed|17853894]